Process History One of the oldest known fermentation products is 1, 3-Propanediol (1,3-PD) (Biebl et al., 1999). As early as 1881, August Freund reliably identified this product in a glycerol-fermenting mixed culture. It contained Clostridium pasteurianum as the active organism. It is a versatile intermediate compound that is used in the synthesis of heterocyclic products. The applications of 1, 3-propanediol is in the field of polymers, such as polyesters and polyurethanes due to the presence of two hydroxyl groups at positions 1 and 3 (Saxena et al., 2009). In 1914, a wine-spoiling bacillus was described by Viosenet which produced the substance, although no comparable strain has been isolated. The quantitative analysis of different enterobacteria that produces 1, 3-PD through fermentation (trimethylene glycol, propylene glycol) started at the microbiology school of Delft, and was successfully continued at Ames, Iowa (Biebl et al., 1999). Interest was shifted during the 1960’s t